ngs amplicon sequencing data Search Results


ngs library quantification kit  (Toyobo)
94
Toyobo ngs library quantification kit
Ngs Library Quantification Kit, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pmc12271030-98-7-11?v=Toyobo
Average 94 stars, based on 1 article reviews
ngs library quantification kit - by Bioz Stars, 2026-07
94/100 stars
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generation sequencing ngs dna amplicon libraries  (Thermo Fisher)
99
Thermo Fisher generation sequencing ngs dna amplicon libraries
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Generation Sequencing Ngs Dna Amplicon Libraries, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pmc09508813-71-0-13?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
generation sequencing ngs dna amplicon libraries - by Bioz Stars, 2026-07
99/100 stars
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nextseq 500  (Illumina Inc)
98
Illumina Inc nextseq 500
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Nextseq 500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pmc09946174-151-13-12?v=Illumina+Inc
Average 98 stars, based on 1 article reviews
nextseq 500 - by Bioz Stars, 2026-07
98/100 stars
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end ngs amplicon sequencing  (Azenta)
86
Azenta end ngs amplicon sequencing
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
End Ngs Amplicon Sequencing, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pmc12490065-111-0-6?v=Azenta
Average 86 stars, based on 1 article reviews
end ngs amplicon sequencing - by Bioz Stars, 2026-07
86/100 stars
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amplicon ez based ngs analysis  (Azenta)
86
Azenta amplicon ez based ngs analysis
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Amplicon Ez Based Ngs Analysis, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pm41720881-320-10-13?v=Azenta
Average 86 stars, based on 1 article reviews
amplicon ez based ngs analysis - by Bioz Stars, 2026-07
86/100 stars
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truseq custom amplicon kit  (Illumina Inc)
95
Illumina Inc truseq custom amplicon kit
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Truseq Custom Amplicon Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pm24694336-38-8-12?v=Illumina+Inc
Average 95 stars, based on 1 article reviews
truseq custom amplicon kit - by Bioz Stars, 2026-07
95/100 stars
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amplicon based library generation  (Illumina Inc)
99
Illumina Inc amplicon based library generation
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Amplicon Based Library Generation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pm40511599-22-10-18?v=Illumina+Inc
Average 99 stars, based on 1 article reviews
amplicon based library generation - by Bioz Stars, 2026-07
99/100 stars
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pan-genotype cdv-specific amplicon-based ngs method  (Oxford Nanopore)
90
Oxford Nanopore pan-genotype cdv-specific amplicon-based ngs method
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Pan Genotype Cdv Specific Amplicon Based Ngs Method, supplied by Oxford Nanopore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/10__1016_slash_j__ijid__2024__107531-73-7-12?v=Oxford+Nanopore
Average 90 stars, based on 1 article reviews
pan-genotype cdv-specific amplicon-based ngs method - by Bioz Stars, 2026-07
90/100 stars
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ngs  (Azenta)
86
Azenta ngs
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Ngs, supplied by Azenta, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pmc12956344-87-9-11?v=Azenta
Average 86 stars, based on 1 article reviews
ngs - by Bioz Stars, 2026-07
86/100 stars
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ngs amplicons  (Wyzer Biosciences)
90
Wyzer Biosciences ngs amplicons
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Ngs Amplicons, supplied by Wyzer Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pmc06512909-429-19-11?v=Wyzer+Biosciences
Average 90 stars, based on 1 article reviews
ngs amplicons - by Bioz Stars, 2026-07
90/100 stars
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16s v3 v4 amplicon sequencing  (Zymo Research)
99
Zymo Research 16s v3 v4 amplicon sequencing
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
16s V3 V4 Amplicon Sequencing, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pm40763732-604-5-11?v=Zymo+Research
Average 99 stars, based on 1 article reviews
16s v3 v4 amplicon sequencing - by Bioz Stars, 2026-07
99/100 stars
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xt index kit  (Illumina Inc)
99
Illumina Inc xt index kit
Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent <t>DNA</t> off-target editing was analyzed by <t>NGS.</t> The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger <t>sequencing</t> results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.
Xt Index Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ngs+amplicon+sequencing+data/pmc06813374-156-12-15?v=Illumina+Inc
Average 99 stars, based on 1 article reviews
xt index kit - by Bioz Stars, 2026-07
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Image Search Results


Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent DNA off-target editing was analyzed by NGS. The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger sequencing results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.

Journal: Nucleic Acids Research

Article Title: Small-molecule activators specific to adenine base editors through blocking the canonical TGF-β pathway

doi: 10.1093/nar/gkac742

Figure Lengend Snippet: Off-target editing induced by SB505124. ( A–D ) Guide RNA-dependent DNA off-target editing was analyzed by NGS. The conversion frequencies of on-target editing at the indicated position (A), fraction of adenine substitutions (B) and off-target editing at three validated OT sites (C) for VEGFA3 are shown. The average off-target editing rate for all OT sites tested was then analyzed (D). ( E ) Schematic diagram of the R-loop assay to detect gRNA-independent DNA off-target editing. ( F ) Bar graphs showing the editing efficiencies of five R-loop regions. ( G, H ) Bar graphs showing the frequencies of A-to-I conversion rates at ABE_OF1 and TOPRS sites in mRNA transcripts (G). Sanger sequencing results show the editing on genomic loci (left panel) and their RNA transcripts (right panel) for ABE_OF1 and TOPRS sites (H). ( I ) NGS results showing the off-target editing frequencies of four mRNA transcripts by ABE 7.10 with or without SB505124. Data are presented as the mean ± SD and are the results of three technical replicates. P -values were determined using a two-tailed Student's t -test.

Article Snippet: Next-generation sequencing (NGS) DNA amplicon libraries were established using Phusion Plus DNA Polymerase (ThermoFisher) and target site primer containing an adaptor sequence (forward: 5′-TTCCCTACACGACGCTCTTCCGATCT-3′, reverse: 5′-AGTTCAGACGTGTGCTCTTCCGATCT-3′) at the 5′ end ( ).

Techniques: Sequencing, Two Tailed Test